It’s been a classroom favorite for over two decades, the classic introduction to biochemistry that brings clarity and coherence to an often unwieldy discipline, a textbook always made fresh by combining the field’s definitive advances with an uncanny ability to reach students.

The quantitative analysis of biological sequence data is based on methods from statistics coupled with efficient algorithms from computer science. Algebra provides a framework for unifying many of the seemingly disparate techniques used by computational biologists.Показать полностьюThe quantitative analysis of biological sequence data is based on methods from statistics coupled with efficient algorithms from computer science. Algebra provides a framework for unifying many of the seemingly disparate techniques used by computational biologists. This book offers an introduction to this mathematical framework and describes tools from computational algebra for designing new algorithms for exact, accurate results. These algorithms can be applied to biological problems such as aligning genomes, finding genes and constructing phylogenies. The first part of this book consists of four chapters on the themes of Statistics, Computation, Algebra and Biology, offering speedy, self-contained introductions to the emerging field of algebraic statistics and its applications to genomics. In the second part, the four themes are combined and developed to tackle real problems in computational genomics. As the first book in the exciting and dynamic area, it will be welcomed as a text for self-study or for advanced undergraduate and beginning graduate courses.

This collection of contributed chapters addresses the mathematical modeling and numerical simulation of liquid capsules and biological cells. Capsules and cells are distinguished from common bubbles and liquid droplets in that their interfaces exhibit mechanical properties that are more involved than those described by a constant and uniform surface tension.Показать полностьюThis collection of contributed chapters addresses the mathematical modeling and numerical simulation of liquid capsules and biological cells. Capsules and cells are distinguished from common bubbles and liquid droplets in that their interfaces exhibit mechanical properties that are more involved than those described by a constant and uniform surface tension. For example, non-uniformities in temperature or interfacial concentration of an insoluble surfactant are responsible for thermocapillary and mass transfer effects that activate the interfaces, in the sense of empowering them with a driving force that contributes to the overall fluid motion, with important consequences and ramifications. The most complex types of particles considered inbthis book are liquid capsules and biological cells enclosed by structured interfaces with a molecular or shell-like constitution, exhibiting viscoelastic properties under direct mechanical or hydrodynamic loads.

This interdisciplinary book gives a comprehensive survey of the state-of-the-art: from applications and trends in fluorescence techniques in science to medicine and engineering.Показать полностьюThis interdisciplinary book gives a comprehensive survey of the state-of-the-art: from applications and trends in fluorescence techniques in science to medicine and engineering. The volume is intended for practitioners and researchers in industry and academia. It covers fields like environmental and materials science, biology, medicine, physics and chemistry. Moreover, it reports about new and breathtaking methods like ultra-fast time-resolved or single molecule spectroscopy, gives examples of applications in the fields of electroluminescent polymers, visualization of membrane potentials in neurons or fluorescence imaging of the brain are described by internationally renowned authors

Highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences.Показать полностьюHighly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection.

Electron Paramagnetic Resonance (EPR) spectroscopy – also sometimes termed Electron Spin Resonance spectroscopy – has manifold potential uses in biochemistry and medicine. The paramount importance of EPR spectroscopy applied to biological tissues and fluids is that it identifies the changes in redox processes that contribute to disease.

The use of spectroscopic methods to examine biomolecules has a long and rich history. Such methods have the virtue of being largely noninvasive and capable of probing living materials as well as subcellular preparations and isolated biomolecules.Показать полностьюThe use of spectroscopic methods to examine biomolecules has a long and rich history. Such methods have the virtue of being largely noninvasive and capable of probing living materials as well as subcellular preparations and isolated biomolecules. The information gained is interpretable in terms of structural parameters and intramolecular interactions. Using time-resolved approaches, dynamics can be explored readily over a time range from less than a picosecond to seconds and longer. This permits ready exploration of intermolecular interactions and intramolecular motion relevant to biological processes.

When it comes to pinpointing the stuff you really need to know, nobody does it better than CliffsNotes. This fast, effective tutorial helps you master the core concepts of biochemistry -- and get the best possible grade.Показать полностьюWhen it comes to pinpointing the stuff you really need to know, nobody does it better than CliffsNotes. This fast, effective tutorial helps you master the core concepts of biochemistry -- and get the best possible grade. From basic biochemical structures to basic interactions, you get clear, jargon-free explanations of all the fundamentals, illustrated throughout with a wealth of figures and formulas.

When it comes to pinpointing the stuff you really need to know, nobody does it better than CliffsNotes. This fast, effective tutorial helps you master the core concepts of Biochemistry II -- and get the best possible grade.Показать полностьюWhen it comes to pinpointing the stuff you really need to know, nobody does it better than CliffsNotes. This fast, effective tutorial helps you master the core concepts of Biochemistry II -- and get the best possible grade. From biosynthesis and integrated metabolism to the molecular cloning of DNA, you get clear, jargon-free explanations of all the fundamentals, illustrated throughout with a wealth of figures and formulas.

The present work is an experimental investigation of high pressure Diesel fuel sprays injected into inert environments under evaporating and non evaporating conditions at high gas pressures. The results further significantly increase current knowledge and understanding of the fundamental processes governing the breakup and atomization of these transient, high pressure fuel sprays and represent a detailed and solid database for the development and testing of spray models in computational fluid dynamics codes.Показать полностьюThe present work is an experimental investigation of high pressure Diesel fuel sprays injected into inert environments under evaporating and non evaporating conditions at high gas pressures. The results further significantly increase current knowledge and understanding of the fundamental processes governing the breakup and atomization of these transient, high pressure fuel sprays and represent a detailed and solid database for the development and testing of spray models in computational fluid dynamics codes. A high temperature and high pressure constant volume cell was used as experimental device. This combustion cell provides a flexible facility to study sprays over a wide range of environments, including conditions found in Diesel engine combustion chambers. It is equipped with an easy configurable Common Rail Diesel injection system. Two complementary measurement techniques where used: The shadow imaging method in combination with appropriate image processing allows the measurement of the axial and radial penetration as a function of time. It also permits the visualization of structures in the liquid phase of the instantaneous spray. The Phase Doppler Anemometry technique (PDA) measures simultaneously the velocity and size of individual droplets at single locations in the fuel spray. Scanning the measurement point over a grid of locations in the spray allows the characterization of the droplet flow field structure in the fuel spray. The following parameter where varied in the experiments of this study: Gas pressure and gas temperature, fuel injection pressure, diameter and length to diameter ratio of the injection nozzle holes (the injector nozzles had always one central hole). This allowed for experiments with up to 96 parameter variations. The measurements of the axial fuel spray penetration over the wide range of gas conditions in this study show the limitations of the corresponding models found in the literature. Most importantly, the influence of the gas density on the initial linear expansion and the influence of the gas temperature on the later propagation of the fuel spray need to be taken into account. Experimental correlations for the fuel spray propagation that have been developed under non evaporating conditions have only a limited usefulness for the estimation of the fuel spray penetration under real diesel engine conditions. The Phase Doppler Anemometry measurements give for non evaporating conditions an average droplet diameter of 8?m ($D_{32} ~ 13mu m$), whereas under evaporating conditions the droplet diameters are reduced by half to sizes of 4?m and less ($D_{32} < 5mu m$). The experimental data in this study could not be interpreted correctly without taking into account the limitations of the applied measurement techniques. The use of two complementary measurement techniques in the same experiment allows for an easier interpretation of the phenomena found in the measurement data. It is shown as well that the manufacturing quality, i.e. the small differences in the micro geometry of the nozzles, may have an even bigger influence on the fuel spray characteristics than the variation of the nozzle hole dimensions itself.

Expert investigators describe in detail the most widely used techniques in chemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo.Показать полностьюExpert investigators describe in detail the most widely used techniques in chemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience. Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today’s gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins.

Peptides play a decisive role in many physiological processes, whether as neurotransmitters, hormones or antibiotics. The rapid developments in peptide research over the past few decades make it almost impossible for newcomers to gain an overview. This means an easily comprehensible yet concise introduction is vital.

This text covers abstract principles of molecular computing and the building of actual systems. Topics include the use of proteins and other molecules for information processing, molecular recognition, computation in nonlinear media, and more.

This comprehensive reference covers the basic knowledge of the regulation of biosynthesis of various amino acids in plants and the application of this knowledge to the discovery of novel inhibitors of amino acid biosynthesis and for enhancing the nutritional value of plant products.Показать полностьюThis comprehensive reference covers the basic knowledge of the regulation of biosynthesis of various amino acids in plants and the application of this knowledge to the discovery of novel inhibitors of amino acid biosynthesis and for enhancing the nutritional value of plant products. Provides an exhaustive list of pathway inhibitors! Synthesizing the research of over 35 contributors worldwide, Plant Amino Acids

Invaluable to chemical and environmental engineers as well as process designers, Chemical Process and Design Handbook shows you how to control chemical processes to yield desired effects efficiently and economically.Показать полностьюInvaluable to chemical and environmental engineers as well as process designers, Chemical Process and Design Handbook shows you how to control chemical processes to yield desired effects efficiently and economically. The book examines each of the major chemical processes, such as reactions, separations, mixing, heating, cooling, pressure change, and particle size reduction and enlargement -- in logically arranged alphabetical chapters, providing you with an understanding of the essential qualitative analysis of each. The Handbook, from expert James Speight:

Leading researchers concisely summarize their hands-on experiences and methods for successfully using the most popular sequence analysis software packages available.Показать полностьюLeading researchers concisely summarize their hands-on experiences and methods for successfully using the most popular sequence analysis software packages available. These experts demonstrate how to examine the data produced by modern automated sequencers, how to assess its quality, how to remove extraneous data, how to align multiple overlapping sequence fragments for either assembly into sequence contigs or comparison with similar sequences from different sources. Procedures for comparing newly derived sequences with the massive amounts of information in the sequence databases are fully covered, as are techniques for performing restriction analysis, searching for open reading frames, calculating the translation products of open reading frames, and making detailed analyses of the expressed “proteins.”

This second edition is a well-rounded, up-to-date handbook of fermentation and biochemical engineering presenting the latest techniques for the commercial production of chemicals and pharmaceuticals via fermentation. Emphasis is given to unit operations fermentation, separation, purification, and recovery. Principles, process design, and equipment are detailed. Environment aspects are covered.

This book presents a lucid account of physical and mathematical approaches to the fundamental problems of evolutionary biology. The first part treats the interrelations of physics and biology, criticizing in particular the so-called anti-reductionist school.Показать полностьюThis book presents a lucid account of physical and mathematical approaches to the fundamental problems of evolutionary biology. The first part treats the interrelations of physics and biology, criticizing in particular the so-called anti-reductionist school. Ideas such as gradualism and punctualism in evolution are described and analysed. The second part is devoted to the molecular foundations of evolution: structure and dynamics of proteins, molecular drive, noncoding DNA. Finally, the problems of evolutionary biology are treated on the basis of synergetics and information theory. In the 1930s Darwin’s theory of evolution was united with population genetics. The present book is the first attempt to unify this longstanding framework with our modern knowledge of molecular biology.

Electroporation Protocols for Microorganisms is the first complete guide to the electroporation of nearly all microorganisms of importance used in biological and biomedical research.Показать полностьюElectroporation Protocols for Microorganisms is the first complete guide to the electroporation of nearly all microorganisms of importance used in biological and biomedical research. It includes reproducible protocols for diverse bacterial, fungal, and protist species-many of which are important in human disease-as well as literature references to electroporation protocols for related species. The contributors also discuss electroporation theory and instrumentation, making it possible to develop new protocols or modify existing ones, and they provide extensive details about culturing and storing many species in a manner designed to optimize electroporation efficiency. Electroporation Protocols for Microorganisms is an indispensable resource for molecular geneticists working directly with microorganisms and for those who employ microorganisms to prepare materials for later introduction into higher organisms, such as plants and animals. Two companion volumes will follow: Plant Cell Electroporation and Electrofusion Protocols and Animal Cell Electroporation and Electrofusion Protocols.

Chemical cross-linking reagents have attained great practical use in industry as well as in basic research, and an understanding of their fundamental principles of reaction is paramount to their applications.Показать полностьюChemical cross-linking reagents have attained great practical use in industry as well as in basic research, and an understanding of their fundamental principles of reaction is paramount to their applications. With broad coverage of the development and application of these reagents, Chemistry of Protein Conjugation and Cross-Linking discusses the mechanism of reaction and allows you to put the theory into practice.