This greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles.Показать полностьюThis greatly enhanced second edition introduces 60 critically important new chapters dealing with topics and techniques that have emerged since the first edition, as well as significantly updating the remaining articles. The new chapters cover with many rapidly developing areas, particularly the application of mass spectrometry in protein characterization, as well as the now well-established 2-D PAGE technique in proteomics. The section on glycoprotein analysis has also been significantly expanded, and methods for the production of single-chain and phage-displayed antibodies have been added to the section on antibodies. Each readily reproducible method follows the highly praised format of the Methods in Molecular Biology™ series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls and on modifying the method to function within your own experimental circumstances.

Since the 1970s the cognitive sciences have offered multidisciplinary ways of understanding the mind and cognition. The MIT Encyclopedia of the Cognitive Sciences (MITECS) is a landmark, comprehensive reference work that represents the methodological and theoretical diversity of this changing field.

В словаре в алфавитном порядке приведены наиболее часто встречающиеся биохимические, физиологические и химические термины, дано их толкование.

В книге представлены основные биологические термины.

Краткий справочник американо-британских соответствий содержит более 1000 слов и выражений, относящихся к наиболее важным темам повседневного общения. Дано описание фонетических, орфографических, лексических и грамматических особенностей американского варианта английского языка в сопоставлении с британским вариантом.

В словаре в алфавитном порядке приведены наиболее часто встречающиеся биохимические, физиологические и химические термины, дано их толкование. Для практических врачей всех специальностей, врачей-интернов, студентов мединститутов, учащихся медучилищ.

Энциклопедический словарь медицинских терминов

Over one hundred well-respected scientists comprehensively review every aspect of the biology underlying ovarian cancer and present a remarkably detailed collection of easy-to-follow methods for illuminating basic mechanisms underlying all forms of cancer.Показать полностьюOver one hundred well-respected scientists comprehensively review every aspect of the biology underlying ovarian cancer and present a remarkably detailed collection of easy-to-follow methods for illuminating basic mechanisms underlying all forms of cancer. Here today’s investigators will find the broadest imaginable array of molecular biology techniques specially designed to determine not only tumor genetics, expression, and protein function, but also to elucidate the genetic mechanisms by which gene and immunotherapies may be perfected. Versatile and comprehensive, Ovarian Cancer Methods and Protocols constitutes a veritable treasury of well-proven expert methods that will help basic scientists and clinical researchers alike master the powerful technologies required for effective cancer research today.

A comprehensive and authoritative treatise on cancer in all of its aspects--from causation and the molecular biological understanding of the nature of cancer, through the principles that underlie all the disciplines necessary for effective diagnosis and treatment, together with a detailed description of all the types of clinical cancer.

Current Protocols in Bioinformatics is the only publication that responds to the need for both a current and updateable source of bioinformatics methodology. This unique publication assures that you have access to a full range of bioinformatics protocols written by globally-recognized experts in the field, and that these protocols are updated and revised as new developments and innovations occur.

"Very comprehensive…Carries on the long tradition of excellence that has sustained it as a standard in its field for many years." (Journal of the American Board of Family Practice, review of 15th Edition).Показать полностью"Very comprehensive…Carries on the long tradition of excellence that has sustained it as a standard in its field for many years." (Journal of the American Board of Family Practice, review of 15th Edition). "Highly recommended." (JAMA, review of 16th Edition). The 17th Edition of this best-selling, classic text provides state-of-the-art coverage of all medical and surgical disorders in children. Readers will find complete, authoritative information on genetics, endocrinology, etiology, epidemiology, pathology, pathophysiology, clinical manifestations, diagnosis, prevention, treatment, and prognosis. Several new contributors have been added, and the text has been revised and updated, including substantial revisions to sections on allergic disorders, the upper and lower respiratory tracts, neoplastic diseases and tumors, diabetes mellitus in children, neuromuscular disorders, behavioral and psychologic disorders, and the ear. This classic text has been enhanced with redrawn artwork, additional tables and boxes to highlight key points, and an enhanced two-color design.

Internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria.Показать полностьюInternationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay).

PATTY'S has become one of Wiley’s flagship publications in occupational health and safety, and the toxicology volumes give proof to the growth and development of the field of toxicology.Показать полностьюPATTY'S has become one of Wiley’s flagship publications in occupational health and safety, and the toxicology volumes give proof to the growth and development of the field of toxicology. What began as a single volume devoted to the field with the first edition (1948) of Patty’s has now mushroomed into eight. This Fifth Edition will permit us to bring about many badly needed changes to the format and organization of the toxicology volumes. In addition to standardizing the format and sequence in which toxicologic data is presented for all of the compounds, the compounds will be organized according to logical groupings, e.g., the metals will be covered in 23 separate chapters making up Volumes II and III; Vol. IV will contain four chapters on aromatic hydrocarbons and 7 chapters on organic nitrogen compounds; Vol. V will contain eight chapters on organic halogenated hydrocarbons and four on aliphatic carboxylic acids; Vol. VI will feature three chapters on ketones, two on alcohols, and five on esters; and Vol. VII will include four chapters on epoxy compounds, two on gycol ethers, and eight on synthetic polymers. The reorganization of chapters in Volumes II through VI by itself will vastly facilitate information searching and retrieval. Volume VIII, like Volume I, does not cover compounds but rather other major issues in toxicology assessment or other forms of toxic agents.

Eminently readable, completely current, and dramatically revised, the new fifteenth edition of Harrison’s PRINCIPLES OF INTERNAL MEDICINE raises the bar for internal medicine references.Показать полностьюEminently readable, completely current, and dramatically revised, the new fifteenth edition of Harrison’s PRINCIPLES OF INTERNAL MEDICINE raises the bar for internal medicine references. Featuring over 90 completely new chapters, HARRISON'S continues to provide the most authoritative record of internal medicine as practiced by the leading experts in the field. And it gives you the one comprehensive database that covers the full spectrum of issues you need to know NOW — basic science, pathophysiology, presenting clinical signs, diagnosis, and the latest treatment guidelines.

Leading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors.Показать полностьюLeading clinical and laboratory scientists describe cutting-edge methods for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumors. The protocols contain detailed instructions and extensive troubleshooting tips that allow researchers effectively to study a wide variety of drug resistance mechanisms, including aspects of drug-induced cell death, drug uptake/efflux, drug metabolism, and DNA repair. Each method is designed to help identify the correlation between molecular and biochemical data and the clinical responses of the patient. Cytotoxic Drug Resistance Mechanisms illuminates all the clinically relevant mechanisms used as markers of the biological response to anticancer biotherapeutics today.

This book not only surveys the whole arena of novel anticancer drug targets, but also presents a wide-ranging selection of cutting-edge techniques currently being applied throughout novel antitumor drug discovery and development.Показать полностьюThis book not only surveys the whole arena of novel anticancer drug targets, but also presents a wide-ranging selection of cutting-edge techniques currently being applied throughout novel antitumor drug discovery and development. The methods are applied to experiments involving such topics as immunotherapy, angiogenesis, cancer metastasis, the cell cycle, signal transduction inhibitors, apoptosis, antibodies, antisense molecules, microarray gene expression, flow cytometry, and PET imaging for cancer target validation. There are also proven methods for the preclinical identification of drug targets and for target validation during the clinical trials of novel agents.

Katherine Bushby and Louise Anderson assemble an outstanding collection of key techniques for the analysis of DNA and protein from patients suspected to suffer from muscular dystrophy.Показать полностьюKatherine Bushby and Louise Anderson assemble an outstanding collection of key techniques for the analysis of DNA and protein from patients suspected to suffer from muscular dystrophy. Each method is highly detailed to ensure success and is presented by a hands-on expert. The various DNA techniques focus on both the X-linked muscular dystrophies and the autosomal recessive muscular dystrophies. The protein methods include expression analysis, multiplex western blot analysis, immunocytochemical analysis, and reviews of immunological reagents and of amplification systems. Comprehensive and highly practical, Muscular Dystrophy: Methods and Protocols offers today’s diagnostic laboratories, basic and medical researchers, and clinicians an authoritative collection of tools that will serve as exacting diagnostic tools as well as greatly empowering research on the novel therapeutics now beginning to emerge.

Andrew Pollard and Martin Maiden bring together a panel of leading authorities to produce a comprehensive, interdisciplinary survey of recent advances and knowledge about meneigococcal disease.Показать полностьюAndrew Pollard and Martin Maiden bring together a panel of leading authorities to produce a comprehensive, interdisciplinary survey of recent advances and knowledge about meneigococcal disease. The review and methods-based chapters collected here provide essential information for diagnosis in the clinical microbiology laboratory, isolate characterization, clinical management, and control of meningococcal disease. They also examine the immunopathological mechanisms occurring in the acutely ill, host–pathogen interactions, and the possible components of meningococcus responsible for virulence. Meningococcal Disease: Methods and Protocols integrates the basic science, clinical, and epidemiological aspects of this terrible infectious disease to promote the deeper understanding needed to identify novel targets for therapeutic interventions and vaccines.

K. Fan Chung and Ian Adcock bring together the first collection of laboratory methods for studying the molecular and cellular mechanisms of asthma. Leading experts describe highly effective methods for obtaining cells from asthmatic airways, analyzing gene and protein expression in these clinical samples, and using molecular and cellular tools for studying cytokine expression and release in this disease.Показать полностьюK. Fan Chung and Ian Adcock bring together the first collection of laboratory methods for studying the molecular and cellular mechanisms of asthma. Leading experts describe highly effective methods for obtaining cells from asthmatic airways, analyzing gene and protein expression in these clinical samples, and using molecular and cellular tools for studying cytokine expression and release in this disease. There are also cutting-edge techniques for studying asthma-related genes and genetic polymorphisms, and for understanding the effects of asthma treatment. User-friendly and state-of-the-art, Asthma: Mechanisms and Protocols provides all asthma researchers-whether novice or experienced, whether in experimental or clinical research-with a first-class collection of readily reproducible pharmacological, cellular, molecular, biochemical, and genetic methods for elucidating not only the mechanisms of the disease itself, but also of the drugs for asthma’s treatment.

The use of mathematical modeling techniques in biomedical research is playing an increasingly important role in the understanding of the pathophysiology of disease processes.Показать полностьюThe use of mathematical modeling techniques in biomedical research is playing an increasingly important role in the understanding of the pathophysiology of disease processes. This includes not only understanding mechanisms of physiological processes, but also diagnosis and treatment. In addition, its introduction in the study of genomics and proteomics is key in understanding the functional characteristics of gene expression and protein assembly and secretion. Finally, with the increasing complexity and associated cost of drug development, modeling techniques are being used to streamline the process. This book is designed to give the reader the mathematical and statistical information necessary to design tracer kinetic studies, to use noncompartmental methodologies and/or to develop multicompartmental models to interpret the data.