Наиболее полная современная сводка о поведении животных, написанная одним из ведущих исследователей в области этологии, внесшим крупный вклад в разработку как экспериментальных, так и теоретических аспектов этой науки.Показать полностьюНаиболее полная современная сводка о поведении животных, написанная одним из ведущих исследователей в области этологии, внесшим крупный вклад в разработку как экспериментальных, так и теоретических аспектов этой науки. В ней на основе сочетания данных сравнительной психологии и физиологии описаны практически все аспекты индивидуального поведения животных. В отдельных главах рассмотрены основные факторы, определяющие ведущие формы поведения, организация восприятия, его избирательность, развитие поведения в онтогенезе, соотношение врожденного и приобретенного в поведении. Книга написана простым языком, что делает ее доступной для читателей-неспециалистов. Предназначена для биологов различных специальностей, для математиков, физиков, инженеров, занимающихся проблемами бионики и кибернетики, для читателей различных специальностей, интересующихся жизнью животных.

В монографии известного американского специалиста по сравнительной физиологии, автора переведенного ранее учебника «Физиология животных. Приспособление и среда» (М.: Мир, 1982) рассмотрена проблема количественных соотношений между размерами животных и физиологическими, а также морфологическими параметрами.Показать полностьюВ монографии известного американского специалиста по сравнительной физиологии, автора переведенного ранее учебника «Физиология животных. Приспособление и среда» (М.: Мир, 1982) рассмотрена проблема количественных соотношений между размерами животных и физиологическими, а также морфологическими параметрами. Книга насыщена богатым фактическим материалом, зачастую очень свежим, изящна по форме изложения, доступна для самого широкого круга читателей. Предназначена для специалистов по сравнительной физиологии, широкого круга биологов и лиц, интересующихся проблемами биологии.

The astounding diversity of the immune system and the complexity of its regulatory pathways makes immunology a combinatorial science. Computational analysis has therefore become an essential element of immunology research and this has led to the creation of the emerging field of immunoinformatics. This book is the first to feature thorough coverage of this new field.

The 2nd edition of this popular text emphasizes the fundamental concepts and principles of human immunology that students need to know, without overwhelming them with extraneous material.Показать полностьюThe 2nd edition of this popular text emphasizes the fundamental concepts and principles of human immunology that students need to know, without overwhelming them with extraneous material. It leads the reader to a firm understanding of basic principles, using full-color illustrations; short, easy-to-read chapters; color tables that summarize key information clinical cases; and much more-all in a conveniently sized volume that’s easy to carry. The New Edition has been thoroughly updated to reflect the many advances that are expanding our understanding of the field. The smart way to study! Elsevier titles with STUDENT CONSULT will help you master difficult concepts and study more efficiently in print and online! Perform rapid searches. Integrate bonus content from other disciplines. Download text to your handheld device. And a lot more. Each STUDENT CONSULT title comes with full text online, a unique image library, case studies, USMLE style questions, and online note-taking to enhance your learning experience.

The fourth edition of this highly popular book has been extensively revised. The first chapter, a revision of the immune system, has been updated to provide the 'nitty-gritty' a student or trainee needs to know in order to understand diseases that have an immunological basis.Показать полностьюThe fourth edition of this highly popular book has been extensively revised. The first chapter, a revision of the immune system, has been updated to provide the 'nitty-gritty' a student or trainee needs to know in order to understand diseases that have an immunological basis. Although the remainder of the book’s focus is clinical, it covers the underlying pathophysiology of disease so that the reader is left with a lasting understanding of the basis of symptoms, signs, special investigations and management of the patient. It will be of great value to medical students and postgraduate trainees who require a thorough knowledge of the rapidly expanding number of diseases that have an immunological basis.

The rapid acceptance of immunohistochemistry as an important and even indispensable adjunct to morphological examination and diagnosis requires the modern anatomical pathology laboratory to be conversant with, and proficient in, immunostaining procedures, as well as methods of tissue processing and antigen retrieval and the underlying characteristics of the increasing number of antibodies and antisera available.

Derek T. O'Hagan and a team of expert vaccinologists and pharmacologists thoroughly describe the preparation, characterization, and evaluation of a wide range of alternative vaccine adjuvants for use in preclinical studies.Показать полностьюDerek T. O'Hagan and a team of expert vaccinologists and pharmacologists thoroughly describe the preparation, characterization, and evaluation of a wide range of alternative vaccine adjuvants for use in preclinical studies. Each chapter carefully reviews a single adjuvant, and suggests why a specific adjuvant might be preferred for a given antigen, depending on what type of immune response is desired. Alternate adjuvant choices are also presented so that researchers can choose those most efficacious for their specific purpose. Comprehensive and highly practical, Vaccine Adjuvants: Preparation Methods and Research Protocols provides an effective guide to making and using vaccine adjuvants. By closely following directions from the book, today’s researchers will be able optimally to induce specific immune responses against different types of antigens and to selectively manipulate the immune response in a favorable way.

This handbook grew out of the original Cytokine Chemokine Application Manual that was first published in 1997. The original manual was based on BD Biosciences technical publications and presentations and with a tremendous amount of input from customers dealing with immune function studies from a “Genes to Proteins to Cells” perspective.Показать полностьюThis handbook grew out of the original Cytokine Chemokine Application Manual that was first published in 1997. The original manual was based on BD Biosciences technical publications and presentations and with a tremendous amount of input from customers dealing with immune function studies from a “Genes to Proteins to Cells” perspective. The new title for this publication reflects the enlarged scope of the book that served as a guide for applications and reagents designed to study the roles played by cells and the regulatory and effector molecules (ie, biological response modifiers including cytokines, chemokines, inflammatory mediators and their receptors) that mediate inflammation and natural and acquired immunity.

This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines.Показать полностьюThis extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines, and vaccines based on inactivated toxins, peptides, and polysaccharide conjugates. New to this edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. There are also chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials.

Expert practitioners present a comprehensive approach to vaccine development, production, and assessment that can be adapted successfully to any vaccine.Показать полностьюExpert practitioners present a comprehensive approach to vaccine development, production, and assessment that can be adapted successfully to any vaccine. Their approach not only includes state-of-the-art techniques for developing live viral and bacterial vectors, expressing foreign vaccine antigens, and producing peptide and conjugate vaccines, but also offers advice and guidance on clinical trials and regulatory requirements. Additional techniques describe such powerful tools as the use of adjuvants, freeze drying vaccines, microencapsulation of vaccines, the full assessment of immunogenicity and potency of vaccines in animal models, and the creation of DNA vaccines, chimeric proteins, and recombinant toxoids.

Pregnancy tests, HIV tests, and tests to confirm heart attacks and many other clinical diagnostic tests are all immunoassays. Without immunoassays a huge variety of biologically important molecules could not be easily detected or quantified.Показать полностьюPregnancy tests, HIV tests, and tests to confirm heart attacks and many other clinical diagnostic tests are all immunoassays. Without immunoassays a huge variety of biologically important molecules could not be easily detected or quantified. Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed.

This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists.Показать полностьюThis much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including “conventional” and recombinant antibodies, fusion proteins, and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.

Highlights recent advances in immunochemical techniques and their impact on basic research and clinical medicine, combining the forces of experts in a wide range of subspecialties to cover a wealth of information.

Luke O'Neill and Andrew Bowie bring together a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction.Показать полностьюLuke O'Neill and Andrew Bowie bring together a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave’s disease, migraine, and myocardial infarction. Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.

This comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out.Показать полностьюThis comprehensive collection of established antibody phage display protocols features authoritative guidance that will enable the nonspecialist successfully to carry them out. Coverage spans the construction of antibody libraries, the selection of antibody clones with the desired properties, and their modification, expression, and purification. Comprehensive and highly practical, Antibody Phage Display: Methods and Protocols provides biochemists, molecular biologists, and immunologists with a gold-standard reference guide to the successful isolation, modification, and expression of recombinant antibodies using today’s powerful phage display technology.

This comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications.Показать полностьюThis comprehensive collection of recently developed methods for producing new antibody reagents by immunization and recombinant DNA techniques contains ready-to-use protocols that illuminate current areas of research on antibody structure, functions, and applications. The methods can be applied in basic immunological studies involving antibody specificity, catalysis, and evolution, and in the isolation of rare antibodies by phage display technology and the engineering of new antibodies by mutagenesis. They offer insight into new ways of developing clinically useful antibody reagents. Antibody Engineering Protocols constitutes a single-source volume for laboratory investigators who want to minimize extensive literature and methodology searches and to work productively in their fields with reproducible step-by-step protocols.

Experienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses.Показать полностьюExperienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses. These well-tested methods range from cellular assays to some of the most up-to-date molecular approaches for identifying compounds that are active against viral enzymes and the development of viral resistance against drugs currently in use. Timely and comprehensive, Antiviral Methods and Protocols offers today’s researchers in academia, clinical departments, and the pharmaceutical industry the powerful, reproducible, and novel methods needed to evaluate compounds effective against both acute and chronic infections.

A comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells.Показать полностьюA comprehensive collection of optimized methods for using lectins in biomedical research. The techniques-fine-tuned by the researchers who developed them-apply lectins to the analysis of carbohydrates, to the isolation and quantification of glycoproteins, and to living cells. Also included are detailed methods for the separation of human bone marrow cells for clinical use, for the assessment of the biological effects of plant lectins, and for the use of light and electron microscopy in analyzing carbohydrate structures. Extremely useful tools for examining structural changes in glycosylation and their functional consequences for human pathophysiology.

A core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals.Показать полностьюA core collection of diverse cutting-edge techniques for the generation, expression, optimization, and characterization of recombinant antibodies. Readily reproducible protocols for lead generation range from the cloning of human immunoglobulin genes to the selection and generation of human recombinant antibodies by humanization approaches, molecular display technologies and transgenic animals. Procedures are also described on restructuring antibody leads into monovalent, multivalent, and bispecific binding fragments for a wide variety of in vivo applications. State-of-the-art technologies are described for the characterization of antigen-binding affinity and specificity with novel applications in radioimmunotargeting, cancer immunotherapy, drug abuse, and proteomics.

Instant Notes in Immunology provides concise yet comprehensive coverage of immunology at an undergraduate level, providing easy access to the core information in the field. The book covers all the important areas of immunology in a format which is ideal for learning and rapid revision.